Bd facscalibur instructions for use bd biosciencesus. Bd facscalibur flow cytometer from bd biosciences selectscience. A practical guide for using flow cytometry and cell sorting, including extensive discussion on hardware, suppliers, reagents, and software. Facscomp instrument setup software is also included with the system. The bd facscalibur used is an automated, multicolor, bench top flow cytometer. About us fluorescence activation cell sorting is a powerful method to study and purify cells for wide applications in immunology, cell biology and other fields of biology.
One striking difference between the facscanto and older generation machines such as the facscalibur is that this system operates on a pcbased windows xp platform using bd facsdiva software. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, labor. The becton dickinson facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. The optional bd facs loader provides walkway automation using a removable 40tube carousel and.
The ease of use of the bd facscalibur system is enhanced by the bd facstation data management system, which has become one of the most popular, userfriendly data management systems in flow. Martz, the site will now be managed by the purdue university cytometry laboratories. Yoav altman he started working with flow cytometry in 2001. The becton dickinson biosciences facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users. Bd facscalibur bd biosciences, san jose, ca bd facscanto 10color bd biosciences, san jose, ca. Beckman coulter moflo xdp bd lsrfortessa x20 beckman coulter cyan adp bd facscan bd facscalibur flow links protocols about flow cytometry flow cytometry is a technique for counting. Facscalibur the flow cytometry facility at the aaron diamond aids research center supplies stateoftheart capacity for cell analysis and sorting. Research and clinical instumentation flow cytometry bd. Files produced by modern flow cytometers are written in the flow cytometry standard format with an. Designed with multicolor application support in mind, the bd facscalibur flow cytometer provides flexibility to support a wide.
The reagents employed were bd multiset cd3cd4cd8cd45 reagents becton dickinson tritest immunofluorescence reagent package insert. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. The bd facscalibur flow cytometer is an innovative platform used for both cell analysis and cell sorting in a single benchtop unit. Bd facscalibur the flow cytometer for your routine cell analysis needs the bd facscalibur flow cytometry system is another bd first. The bd facstation system automates many software functions and delivers highperformance acquisition and analysis tools for creating plots. The percent fluorescence of pselectin surface expression on platelets was analyzed by facscalibur flow cytometry, and the data analysis was performed with flowjo software flowjo, llc. Facscalibur a uses flowjo collectors edition acquisition software quick start guide users guide as well as the cytek ams software.
A variety of applications are supported by the instrument in large part due to a high degree read more. Using the installed software with the facscalibur flow cytometer, you will. The system is designed with a multicolor application support which allows accommodation of a wide range of laboratory and clinical applications. The bd facscalibur, a fully integrated, multiparameter system, offers the performance and sensitivity needed to ensure the objective and reproducible results vital to laboratories. Combined with your choice of sample loader, a wide variety of reagents, and powerful software, the bd facscalibur system offers the high throughput necessary. Bd cellquest software 3d plot bd attractors software.
Accuri c6 is an analyzer equipped with two lasers and 4 color detectors and it has capabilities similar to facscalibur. Jan 20, 2016 bd facscalibur flow cytometer available at the lab world group. It is a tricolor, automated system that is capable of analyzing a wide variety of. The bd facscalibur platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system. Comparison of flow cytometric methods for the enumeration. Resources flow cytometry core facility boston university. How we support the mission of the flow cytometry and cell sorting core facs2 is to provide researchers at chla. Bd biosciences 2 laser facscalibur flow cytometer marshall. Bd facs calibur microscopy and microanalysis facility. Facs and falcon are registered trademarks of becton dickinson and company. The facscalibur system is a modular, benchtop flow cytometer from becton dickinson immunocytometry system bdis that is easily modified based on research needs. There are three facscalibur instruments in the flowcore facility.
The becton dickinson biosciences facscalibur flow cytometer offers a unique approach to flow cytometry, allowing users to perform both cell analysis and cell sorting in a single system, providing flexibility for a variety of research and clinical applications. Catalog of free flow cytometry software the original cytometry software catalog was developed and managed by dr. Catalog of free flow cytometry software purdue university. Designed specifically to support a wide range of applications, the. Research and clinical instumentation flow cytometry. Characterize complex immunephenotypes and rare cells of interest with our wellestablished flow cytometry platform characterize cells in high throughput with limited sample volume enabled by.
The ease of use of the bd facscalibur system is enhanced by the bd facstation data management system, which has become one of the most popular, userfriendly data management systems in flow cytometry. Analysis can be performed on the bd accuri c6 plus. It is equipped with a blue 488nm laser and a ddm board and data collected include forward scatter, side scatter, fl1 53030nm, fl2 58542nm, and fl3 65030nm. Flow cytometry bosch bosch institute the university. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of. Flow cytometry equipment school of medicine clinical. Bd facscalibur cell analyzer and sorter for fast, easy, and accurate results for routine applications. Bd facscalibur flow cytometer gmi trusted laboratory. Bd bd biedt een waaier van producten voor research and klinische instrumentatie, waaronder. Since the initial commercialization of flow cytometry fc and fluorescence activated cell sorting facs in 1968, they have undergone significant improvements.
Becton dickinson facscalibur flow cytometry system youtube. I already had optimized before the settings for my. To ensure the flow cytometer provides consistent results, run bd facscomp software with bd calibrite beads at the start of each workday. The becton dickinson facscalibur flow cytometer is an innovative single benchtop system, combining cell analysis and employing a customizable approach to flow cytometry analysis through modular. Sensitivity is established thanks to a four color, dual laser optical system that is able to read a number of signals at once. Bd biosciences provides flow cytometers, reagents, tools and a wide range of services to support the work of researchers and clinicians who understand disease and improve care. The becton dickinson facscalibur flow cytometer is an innovative single benchtop system, combining cell analysis and employing a customizable approach to flow cytometry analysis through modular components. Bridging the gap between flow cytometry and results.
In addition, c6 has a flow metering system for automatic calculation of absolute cell. Bd biosciences facscalibur flow cytometer features. Bd accuri c6 plus flow cytometer with its compact 11 x 14. We thank nate sheaffer and joseph bednarczyk from penn state college of medicines flow cytometry core for assistance with flow cytometry analysis and cell sorting.
Instruments and services flow cytometry core facility. In the following quiz, were going to be learning all about the technological advancement that allows us to analyze the physical and chemical characteristics of particles in a fluid while it passes through a laser. Instructions for use manual bd facscalibur instructions for use manual 238 pages flow cytometer. Features of free software packages in flow cytometry. The becton dickinson bd facscalibur is a flow cytometer that provides highly sensitive cell analysis. Flowjo software is a powerful analysis tool for the management, display, manipulation, analysis and publication flow cytometry data, addressing a key challenge in the interpretation of data about bd. The bd facsvia system features novel designs in hardware, software, and instrument qc. Our goal in flow cytometry is to help you achieve your goalsby providing the technology you need to get the most accurate, reproducible results, whether for routine cell based assays or for highcomplexity flow cytometry applications. The mission of the flow cytometry and cell sorting core facs2 is to provide researchers at chla and other institutions with the ability to perform multiparameter analysis and cell sorting on singlecell suspensions to asses cell morphology, phenotype, and function through standard and custom applications in support of biomedical research. About bds research and clinical instrumentation products. Bd biosciences offers innovative software products.
One striking difference between the facscanto and older generation machines such as the facscalibur is that this. Bd biosciences has developed a variety of useful tools and resources that help researchers and clinicians alike to visualize their flow cytometry experiments and assays. Once you select the core, you can select the equipment under the choose a cytometer menu. Flow cytometry and in vivo imaging core laboratory research. The flow cytometry core staff has more than 30 years of cell sorting and flow cytometry experience in ncidesignated cancer center core labs. Our bd facscalibur is a one laser, five detector analysis flow cytometer capable of measuring 3 colors simultaneously. It is the newest generation of the bd accuri platform. Bd facscalibur system is a fully integrated multiparameter system. Beckman coulter moflo xdp bd lsrfortessa x20 beckman coulter cyan adp bd facscan bd facscalibur flow links protocols about flow cytometry flow cytometry is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. The bd facscanto is often hailed as the new generation of flow cytometry. Flowjo is a software package for analyzing flow cytometry data.
The bd facscalibur system is an automated benchtop flow cytometry system that offers three or fourcolor capability and can perform both cellular analysis and optional cell sorting. Instrument received a full pm and is ready for resale. Bd biosciences offers innovative cell sorters and analyzers that support all aspects of research and clinical flow cytometry. Facs data from acquired on facscaliburmac to flowjopc. This instrument is equipped with two lasers 488nm and 635nm and detectors for forward. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. Bd offers a variety of products for research and clinical instrumentation, including. Bd facscalibur instructions for use bd biosciences. Bd biosciences facscalibur flow cytometer features system. Since the initial commercialization of flow cytometry fc and. From turnkey systems for hivaids monitoring to highperformance systems.
Bd cellquest pro bd facscalibur and bdfacsort mac bd facsdiva bd facsaria fusion, bd fcsariaii, bd lsrii, bd faccanto, lsrfortessa pc flowjo mac, pc fcsexpress pc kaluza v1. We have 1 bd facscalibur manual available for free pdf download. May, 2016 becton dickinson facscalibur flow cytometry system with hts autosampler available at the lab world group. Invitrogen introduction to flow cytometry 15 minutes bd bioscience training courses. Bd accuri c6 this is a twolaser 488nm, 640 nm personal flow cytometer located at the blood research institute. The gold standard that continues to evolve the bd facscalibur flow cytometer is another bd biosciences first. We compared the performance of the bd facsvia system using the bd leucocount kit with the bd. Thomas adejumo mass cytometry senior field application. The bd facscalibur platform allows users to perform both cell analysis and cell sorting in a single benchtop system.
The system is designed with a multicolor application support which allows. The facility houses a becton dickinson facscalibur 2. These instructions describe how to operate your bd facscalibur flow cytometer using bd facstation software. Bd is one of the largest global medical technology companies in the world and is advancing the world of health by improving medical discovery, diagnostics and the. Bd biosciences offers innovative software products to support all aspects of flow cytometry and cellular imaging systems. Fcs express gets you from raw data to easilyunderstandable, beautifully formatted, presentationready results more easily and in less time than any other flow cytometry software. We are bostonlocated flow cytometry core facility equipped with analyzers 5laser bd lsr ii sorp, a facscanto and a facscalibur and cell sorters 4 laser16 color facs aria ii sorp and a 5 laser25. Download the cytek ams software to create layouts on your pc and. It designed for applications that range from routine clinical to advanced research, analyzing single cells as they pass through a focused laser beam. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, laborintensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently. Application of flow cytometry in laboratory medicine. Bubmc users are eligible for up to 2035% discount, please email us for the quote code.
Bd biosciences facscalibur flow cytometer services. Facscanto flow cytometer from bd biocompare product. Other software fcs and setup files created within bd facscanto. Bd biosciences 2 laser facscalibur flow cytometer w hts autosampler. Facscalibur a can run samples in a 96well plate with the automated microsampler ams system. Combined with your choice of sample loader, a wide variety of reagents, and powerful software, the bd facscalibur. Flow cytometry and cell sorting penn state college of. This online tool guides you through flow cytometry panel design, providing a simplified, customizable. Bd facscalibur 2 laser, 4 color flow cytometer marshall.
The cbbc houses a bd facscalibur flow cytometer in room l4076. The intuitive bd facscalibur flow cytometer instrument can be upgraded to keep up with the laboratorys sorting and automation needs. Flow cytometry shared resources flow cancer center. Bd facscanto cell analyzer with proven reliability and high quality results. Flow cytometers that identify, count, and characterize cells to support cell analysis. Analysis occurs at the cuvette flow cell, increasing sensitivity a nd resolution, allowing detection of 2 relative physical properties as well as up to 4 different colors. The tabbed interface provides quick access to the collection, analysis and statistics functions. Has a three or fourcolor fluorescence capability and is flexible and modular for future upgrades.
Interference there are no known incompatibilities with bd facscanto clinical. The channel number is transferred to the computer via the general purpose inout. Used becton dickinson facscalibur 2032070 flow cytometry. Flow cytometry bosch bosch institute the university of. Bd, the bd logo, bd calibrite, bd cellquest, bd clonecyt, bd facs, bd facscalibur, bd facscan. Managing a core service including cell sorting, training, data generation and data analysis for flow cytometry bd facscalibur, arias ii, iii and fusion, lsr ii and bd influx and flow microscopy services. Once you have registered at this website, you will find camac flow cytometry core shelby location listed under the university of alabama of birmingham uab. Bd intro to flow training about 1 hour bd facsdiva v6 software overview course 6. Designed with multicolor application support in mind, the bd facscalibur flow cytometer provides flexibility to support a wide variety of research and clinical. Flow cytometry has evolved rapidly to incorporate the use of multiple fluorochromes to effectively identify and isolate, with an optional sorting module, subset. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of lymphocyte subsets, stem cells, residual white blood.
The system supports a wide variety of research and clinical applications and is. Antibodies, buffers and other flow cytometry reagents. The bd facsvia flow cytometry system is an easytouse benchtop solution that helps meet the challenges of rising costs, decreasing reimbursement, labourintensive manual procedures, fewer resources and higher demands for efficiency, so your laboratory runs smoothly and efficiently. Bd biosciences facscalibur flow cytometer product list. Bd accuri c6 plus software has an intuitive user interface. Bd netherlands research en klinische instrumenten flow. The bd facscalibur is a flow cytometer analyzer equipped with 2 lasers, generating data of up to 6 parameters for each event passing through the interrogation point. Evaluation of the partec flow cytometer against the bd.
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